Download Kinetics for the Life Sciences: Receptors, Transmitters and by H. Gutfreund PDF

By H. Gutfreund

ISBN-10: 0511626207

ISBN-13: 9780511626203

ISBN-10: 0521480272

ISBN-13: 9780521480277

ISBN-10: 052148586X

ISBN-13: 9780521485869

This e-book introduces the reader to the kinetic research of quite a lot of organic strategies on the molecular point. It exhibits that a similar method can be utilized to solve the variety of steps for quite a lot of platforms together with enzyme reactions, muscle contraction, visible conception, and ligand binding. the writer discusses the equipment for characterizing those steps in chemical phrases. Firmly rooted in conception, a variety of examples and experimental innovations are brought besides. A old process is used to illustrate the advance of the speculation and experimental ideas of kinetic research in biology.

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Extra info for Kinetics for the Life Sciences: Receptors, Transmitters and Catalysts

Example text

2 Modelling of kinetic mechanisms 29 kinetic mechanisms. Most of the algorithms applied to kinetic problems are based on one mathematical principle, stepping methods which have been progressively refined. Other procedures, such as series expansion and Monte Carlo methods, will be found in texts on numerical analysis. The Monte Carlo method (see for instance Kleutsch & Frehland, 1991) has proved itself particularly useful for the simulation of the growth of individual myosin filaments (Davis, 1993) and microtubule lattices (Martin, Schilstra & Bayley, 1993).

The same quantity is also referred to as a relaxation time (which is quite acceptable jargon and generally used for small perturbations, see chapter 6), or simply as rise time; however the use of decay time is ambiguous and therefore unacceptable. An electronic example Similar equations occur in many contexts. 12) where Ic(t) is the current flowing into the capacitor at time t9 V(t) is the voltage across the capacitor at time t, and Cis the capacitance (measured in farads) which is a constant. 2, which can be taken as a representation of the electrical properties of a cell membrane.

Measuring this time constant (by injecting rectangular current pulses) allows the membrane capacitance to be measured. 01 F m" 2 ). It is, incidentally, clear from this argument that the membrane capacitance can result in problems when trying to measure the time course of currents that flow through a membrane when, for example, ion channels open; the time course of the membrane potential V(t) will not follow accurately the time course of the underlying changes in membrane current. 12), no current can flow into the membrane capacitance; the measured current will therefore represent accurately the opening and shutting of ion channels.

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Kinetics for the Life Sciences: Receptors, Transmitters and Catalysts by H. Gutfreund


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