Download DNA-Protein Interactions: Principles and Protocols, Third by Peter G. Stockley (auth.), Benoît Leblanc, Tom Moss (eds.) PDF

By Peter G. Stockley (auth.), Benoît Leblanc, Tom Moss (eds.)

ISBN-10: 1603270140

ISBN-13: 9781603270144

Gene expression can suggest the adaptation among a practical and non-functional genome, among well-being and ailment, and with the advance of transgenic plants, the adaptation among survival and hunger. In DNA-Protein Interactions: rules and Protocols, 3rd Edition, this very important topic is pointed out thus far with protocols exploring the main state of the art advancements within the box, together with in vivo and genome-wide interplay concepts. Addressing subject matters equivalent to chromatin immunoprecipitation, topological experiences, photocrosslinking, be concerned and imaging options, the quantity absolutely updates and expands upon the winning earlier variants. Written within the handy and informative Methods in Molecular Biology™ sequence layout, chapters contain introductions to their respective matters, lists of the required fabrics and reagents, step by step, with no trouble reproducible laboratory protocols, and notes on troubleshooting and fending off identified pitfalls.

Comprehensive and authoritative, DNA-Protein Interactions: ideas and Protocols, 3rd Edition serves as a terrific consultant for all these exploring this dynamic, crucial, and more and more cheap region of research.

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Extra info for DNA-Protein Interactions: Principles and Protocols, Third Edition

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Biotechniques 16, 590–592. J. A. (1995). Biotinylated probes in the electrophoretic mobility shift assay to examine specific dsDNA, ssDNA or RNA–protein interactions. Nucleic Acids Res. 23, 3792–3793. , Heiser, W. and Jolly, J. (1990). Fast gel electrophoresis to analyze DNA–protein interactions. Biotechniques 8, 556–563. G. B. (1995). Alternative to polyacrylamide gels improves the electrophoretic mobility shift assay. Biotechniques 18, 704–706. W. F. (1998). Use of modified agarose gel electrophoresis to resolve protein–DNA complexes for electrophoretic mobility shift assay.

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2004). The utility of a two-color fluorescence electrophoretic mobility shift assay procedure for the analysis of DNA replication complexes. Electrophoresis 25, 2439–2446. J. and Chu, G. (2006). Electrophoretic mobility shift assays to study protein binding to damaged DNA. Methods Mol. Biol. 314, 323–344. G. A. ) (1992). Short Protocols in Molecular Biology. Wiley, New York, NY. G. L. (1997). Transcriptional regulation of the rat poly(ADPribose) polymerase gene by Sp1. Eur. J. Biochem. 250, 342–353.

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DNA-Protein Interactions: Principles and Protocols, Third Edition by Peter G. Stockley (auth.), Benoît Leblanc, Tom Moss (eds.)

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