By Francesco Albanese
This booklet discusses canines and tom cat epidermis cytology and the significance of this diagnostic device in studying dermis lesions. With greater than six hundred medical and cytological colour photos, it explains the cytological styles saw in all cutaneous inflammatory and neoplastic lesions in cats and canine, in addition to cutaneous metastasis of non-primary epidermis neoplasms. the 1st a part of the publication describes telephone morphology and cytological styles, delivering an summary of the traditional constitution of the surface. within the moment bankruptcy, readers the best way to decide on the easiest thoughts for various kinds of lesions. additional chapters current the cytological findings usually inflammatory and neoplastic dermis ailments. via concentrating on the macroscopic elements of the lesions from which the cells are amassed, it is helping readers to interpret cytological specimens. the ultimate bankruptcy explores the cytology of cutaneous metastasis from inner organs or accent glands. This ebook deals veterinary scholars and practitioners alike a vital diagnostic tool.
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Extra info for Canine and Feline Skin Cytology: A Comprehensive and Illustrated Guide to the Interpretation of Skin Lesions via Cytological Examination
Pustules tend to rapidly dehydrate and give rise to yellowish crusts or epidermal collarettes. In these cases, as described below, it is possible to obtain cytological specimens by collecting the exudate present below the crust at the edges of the collarettes. 50 2 Techniques of Sampling, Preparation and Staining of Cytological Specimens Fig. 12 The Tzanck test: rupture of a large pustule with a needle Fig. 2 Sampling Techniques 51 Fig. 3 Cytological Sampling from Intact Skin Covered with “Dry” or “Oily/Waxy” Scales Scales are aggregates of corneocytes that represent the final product of the physiological keratinisation process of the epidermis and are continuously released into the environment, both singly and in small clusters (Fig.
27). Their presence is rarely important in skin cytology, but allows us to define the depth of the lesions (the epidermis is not vascularised) and can be used as a cytological meter to compare the size of other cells. 2 Neutrophils Neutrophils are the most frequently detected cells in cytological specimens coming from inflammatory skin lesions. Neutrophils leave the bloodstream and, together with other leukocytes, migrate into the dermis in response to chemotactic molecules released from the same cells of the skin or from micro-organisms, parasites, foreign bodies etc.
1 Impression Smears The impression smear is the only executable method we can perform when cells must be sampled from non-raised, superficial “wet” (exudative) lesions. This technique is frequently used because with this method, it is possible to collect cells simply by placing a slide on the lesion. As the cells easily adhere to the slide, it is sufficient to gently place it on the lesion without exerting excessive pressure, which could alter the cell morphology. Nevertheless, there are some basic precautions that differ according to the lesion that must be investigated.
Canine and Feline Skin Cytology: A Comprehensive and Illustrated Guide to the Interpretation of Skin Lesions via Cytological Examination by Francesco Albanese