By Zhenjiang Li, Xiaojun Ji, Suli Kan, Hongqun Qiao, Min Jiang (auth.), G. T. Tsao, Pingkai Ouyang, Jian Chen (eds.)
Past, current, and destiny business Biotechnology in China, via Zhenjiang Li, Xiaojun Ji, Suli Kan, Hongqun Qiao, Min Jiang, Dingqiang Lu, Jun Wang, He Huang, Honghua Jia, Pingkai Ouyuang, and Hanjie Ying; natural chemical substances from Bioprocesses in China, by means of Jin Huang, Lei Huang, Jianping Lin, Zhinan Xu, and Peilin Cen; Biofuels in China, via Tianwei Tan, Jianliang Yu, Jike Lu, and Tao Zhang; Bioreactors and Bioseparation, by means of Siliang Zhang, Xuejun Cao, Ju Chu, Jiangchao Qian, and Yingping Zhuang; Environmental Biotechnology in China, through Shuang Jiang Liu , Lei Liu , Muhammad Tausif Chaudhry , Lei Wang , Ying Guang Chen , Qi Zhou , He Liu , and Jian Chen; conventional chinese language Biotechnology, by means of Yan Xu , Dong Wang , Wen Lai Fan , Xiao Qing Mu, and Jian Chen; smooth Biotechnology in China, via Qing-Zhao Wang and Xue-Ming Zhao
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Extra resources for Biotechnology in China II: Chemicals, Energy and Environment
11 U/mL . The complete sequence encoding the mature peptide of endo-1,4-glucanase II of T. reesei QM9414 was amplified. The gene was inserted into the P. pastoris vector pPIC9K, downstream of the a-factor signal peptide sequence. Then the recombinant plasmid pQY2025 was lineated by BglII digestion and introduced into the host P. pastoris GS115 by electroporation. After screening, the recombinant P. pastoris strain Gp2025 was obtained and fermented in 25-mL methylotrophic culture medium. 00 U/mL.
346 strain (Fermentation Research Institute, No. 11011A-1 (Fermentation Research Institute, No. 1109), No. 50833 strain (Fermentation Research Institute, No. 1110), S. albulus strain no. 410 and the like; this type is commonly capable of producing e-PL with a degree of polymerization between 25 and 35 on a large scale. The other is represented by strains producing middle to low degrees of polymerization of e-PL, for example, S. albulus 20 Z. Li et al. subsp. sp-25 strain’s (FERM P-17998) variant strain, Streptomyces lavendulae USE-53 strain (FERM P-18350), Kitasatospora kifunense strain MN-1 and the like; this type is commonly capable of producing e-poly-L-lysine with a degree of polymerization between 10 and 19 in low yield .
The low yield of cellulase by mutant microorganisms causes the high price. Although the overexpression of cellulase can be carried out by recombinant strains, there is no commercial recombinant cellulase for Chinese bioethanol industry. In the future, development of highly efficient recombinant microbes is feasible to reduce the price of cellulase. Thirdly, highly efficient microorganisms for pentose and hexose cofermentation are in short supply. Efforts must be made to reconstruct strains to utilize pentose and hexose simultaneously and resist the inhibitory effect of alcohol and inhibitors.
Biotechnology in China II: Chemicals, Energy and Environment by Zhenjiang Li, Xiaojun Ji, Suli Kan, Hongqun Qiao, Min Jiang (auth.), G. T. Tsao, Pingkai Ouyang, Jian Chen (eds.)