By Andy Beaumont, Pierre Boudry, Kathrin Hoare(auth.)
Following the super well-received constitution of the 1st version, this rigorously revised and up-to-date re-creation now contains a lot new details of significant significance to these operating and gaining knowledge of within the fisheries and aquaculture industries.
beginning with chapters overlaying genetic version and the way it may be measured, the authors then examine genetic constitution in ordinary populations, by means of a brand new bankruptcy masking genetics in terms of inhabitants dimension and conservation matters. Genetic version of characteristics and triploids and the manipulation of ploidy are absolutely coated, and one other new bankruptcy is integrated, entitled 'From Genetics to Genomics'. The publication concludes with a bankruptcy protecting the impression of genetic engineering in aquaculture.
With the inclusion of a wealth of up to date details, new textual content and figures and the inclusion of a 3rd writer, Pierre Boudry, the second one version of Biotechnology and Genetics in Fisheries and Aquaculture presents a great textual content and reference of serious price and use to higher point scholars and pros operating throughout fish biology, aquatic sciences, fisheries, aquaculture, genetics and biotechnology. Libraries in all universities and study institutions the place organic sciences, fisheries and aquaculture are studied and taught must have numerous copies of this glorious new version on their cabinets.
- Completely up to date, revised and improved re-creation
- Subject region of ever expanding significance
- Expanded authorship
- Commercially important info for fish breeders
Chapter 1 what's Genetic edition? (pages 1–18):
Chapter 2 How can Genetic edition be Measured? (pages 19–48):
Chapter three Genetic constitution in usual Populations (pages 49–76):
Chapter four Genetics of inhabitants dimension in Conservation and Aquaculture (pages 77–97):
Chapter five Genetic version of qualities (pages 99–128):
Chapter 6 From Genetics to Genomics (pages 129–143):
Chapter 7 Triploids and past: Why manage Ploidy? (pages 145–160):
Chapter eight Genetic Engineering in Aquaculture (pages 161–180):
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Extra info for Biotechnology and Genetics in Fisheries and Aquaculture, Second Edition
P1: IFM/UKS BLBK242-02 P2: SFK BLBK242-Beaumont January 4, 2010 23:20 Printer Name: Yet to Come Chapter 2 How can genetic variation be measured? Genetic variation can be measured and quantified at several levels. Firstly, the precise sequence of a DNA fragment, and how it varies between individuals, can be determined. Secondly, differences between sizes of DNA fragments can be identified. At the next level, we can consider protein differences that result from DNA coding sequence variation. Finally, it is sometimes possible to identify phenotypic differences that are the product of genetic variation at just one or two loci or for traits determined by a much larger number of genes.
Each microtube contains a number of ingredients together with the template DNA that is to be copied from. Millions of copies of a pair of primers – short single-strand sequences of DNA each complementary to one end of the target DNA sequence – are included. g. Taq polymerase, derived from the bacterium Thermus aquaticus, a resident of hot springs) is present together with the four deoxynucleotide triphosphates (dATP, dCTP, dGTP and dTTP, collectively dNTPs) in a buffer. Using Taq polymerase, the maximum length of the target DNA is effectively around 3–4 kb, because longer fragments cannot be successfully amplified and P1: IFM/UKS BLBK242-02 P2: SFK BLBK242-Beaumont 24 January 4, 2010 23:20 Printer Name: Yet to Come Biotechnology and Genetics in Fisheries and Aquaculture inaccuracies begin to accumulate to unacceptable levels.
The other group of nucleotide polymorphisms are insertions or deletions of nucleotides, called ‘indels’, and these are sometimes included within the general name of SNPs. Large indels can be identified by scoring fragment length polymorphism or restriction fragment length polymorphism (RFLP), but single-nucleotide indels are less frequent and less informative than true SNPs. 5). 5 Alignment of sequences is used to identify single nucleotide polymorphisms and Indels. 1 = transition, 2 = transversion, 3 = indel.
Biotechnology and Genetics in Fisheries and Aquaculture, Second Edition by Andy Beaumont, Pierre Boudry, Kathrin Hoare(auth.)