Download Biotechnology and Ecology of Pollen: Proceedings of the by M. B. Singh, R. B. Knox (auth.), David L. Mulcahy, Gabriella PDF

By M. B. Singh, R. B. Knox (auth.), David L. Mulcahy, Gabriella Bergamini Mulcahy, Ercole Ottaviano (eds.)

ISBN-10: 1461386225

ISBN-13: 9781461386223

ISBN-10: 1461386241

ISBN-13: 9781461386247

In acceptance of the Forgotten iteration D. L. MULCAHyl Pollen used to be lengthy believed to serve essentially a unmarried functionality, that of supplying male gametes to the egge A secondary and usually ignored worth of pollen is that it serves to dam the transmission of many faulty alleles and gene combos into the following iteration. This latter functionality comes approximately just because pollen tubes wearing faulty haploid genotypes usually fail to accomplish development during the whole size of the fashion. although, the valuable results of this pollen choice are diluted by way of the truth that a similar deleterious genotypes are usually transmitted during the egg at strictly mendelian frequencies (Khush, 1973). Gene expression within the pollen could therefore at the very least seem to be a phenomenon of trivial outcome. certainly, Heslop-Harrison (1979) rightly termed the gametophytic component of the angiosperm existence cycle, the "forgotten generation." This overlook, besides the fact that, happened regardless of sophisticated yet consistent symptoms that pollen is the location of excessive gene job and choice. for instance, Mok and Peloquin (1975) established that really heterozygous diploid pollen exhibits heterotic features while fairly homozygous diploid pOllen doesn't. This used to be facts optimistic that genes are expressed (that is, transcribed and translated) within the pollen. 1 division of Botany, collage of Massachusetts Amherst, MA 01003, united states viii in spite of the fact that, the consequences for pollen biology of even this fresh and renowned learn weren't generally recognized.

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Extra info for Biotechnology and Ecology of Pollen: Proceedings of the International Conference on the Biotechnology and Ecology of Pollen, 9–11 July, 1985, University of Massachusetts, Amherst, MA, USA

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6. Mulcahy OL (1979) The rise of Angiosperms: a genecological factor. Science 206: 20-23. 7. Ottaviano E, Sari Gorla M, l1ulcahy OL (1980) Pollen tube growth rate in Zea mays: implication for genetic improvement of crops. Science 210: 437-438. 8. pfahler PL, Linskens KF, School HW, fvilcox H (1981) Surfactant effects on petunia pollen germination in vitro. Bull Environ Contam Toxicol 26: 567-570. 9. , S. ) Krause and Mimulus guttatus OC. ~heor Appl Genet 69: 597-602. 10. Shields CR, Orton TJ, Stuber CW (1983) An outline of general resource needs and procedures for the electrophoretic separation of active enzymes from plant tissue.

Similar results have been obtained for several other pollen specific clones for both Tradescantia and corn (Mascarenhas et al manuscript in preparation) . r ..... t Figure 1. Relative specific mRNA levels at different stages of Tradescantia pollen development determined by densitometric scans of Northern blots using two pollen specific probes (Tpc44 and Tpc70) and an actin probe (pSAc3). T: tetrads, immediately following meiosis MI: microspore interphase MM: microspore mitosis PI-I: early pollen interphase; the vegetative nucleus becomes diffusewhile the generative nucleus begins to elongate.

14 However, the duplicate loci did not exhibit significant divergence either in pollen or leaf tissue. In no case did the relative expression of duplicate loci differ in pOllen compared to leaf tissue. The differences in isozyme phenotype which were observed could, in general, be explained either by the respective isozyme system (esterase and peroxidase isozymes are known to often display tissue specificity) or by organellar location (TPI). References Adams WT, Joly RJ (1980) Genetics of allozyme variants in loblolly pine.

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Biotechnology and Ecology of Pollen: Proceedings of the International Conference on the Biotechnology and Ecology of Pollen, 9–11 July, 1985, University of Massachusetts, Amherst, MA, USA by M. B. Singh, R. B. Knox (auth.), David L. Mulcahy, Gabriella Bergamini Mulcahy, Ercole Ottaviano (eds.)


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