Download Bioinformatics: A primer by P. Narayanan PDF

By P. Narayanan

ISBN-10: 8122416101

ISBN-13: 9788122416107

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Typically a peptide consists of less than 50 amino acids while a protein has greater than 50 amino acids. Fig. 4 Formation of Polypeptides from Amino Acids via Peptide (C–N) bonds Operationally, the structural and functional features and protein complexes are addressed at four levels of hierarchical structural organization (1) primary structure, (2) secondary structure, (3) tertiary structure and (4) quaternary structure. 1 The Primary Structure (1°-Structure) The linear number and order of the amino acids present in a peptide or protein constitutes the primary structure (1°-structure).

1 – 3 kb). Microchip electrophoresis is an electrophoretic method that has considerable impact in DNA separations, in large part because microchip electrophoresis offers some clear advantages over slab gel electrophoresis for automation, speed, and quantitative capability. 2 Column Electrophoresis Both slab gel and capillary gel (and free-solution) matrices can be employed to separate nucleic acids and proteins. In order to accomplish sizing, a sieving matrix is prepared and loaded either between two glass plates (slab gel), or into glass capillaries.

High Performance Liquid Chromatographic (HPLC) methods are aimed at increasing the efficiency of liquid chromatography to a high level of sensitivity (10–12 g/L). Advent of HPLC has resulted in tremendous progress in the separation of a wide variety of inorganic and organic compounds. Reversedphase HPLC (based on the hydrophobicity of compounds) has become the workhorse for purification and characterization of molecules of biological importance. Denaturing high-performance liquid chromatography (dHPLC) is used in single nucleotide polymorphism (SNP)-detection methods, based on discrimination between perfect and mismatched hybridization, measures melting temperatures.

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Bioinformatics: A primer by P. Narayanan

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