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By Georg Gübitz, Alexander Bauer, Guenther Bochmann, Andreas Gronauer, Stefan Weiss

Michael Lebuhn, Stefan Weiß, Bernhard Munk, Georg M. Guebitz

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Anaerobic Fungi and their power for Biogas Production

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Hygiene and Sanitation in Biogas Plants

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J Bacteriol 176:6107–6119 87. Birch A, Leiser A, Robinson JA (1993) Cloning, sequencing, expression of the gene encoding methylmalonyl-coenzyme A mutase from Streptomyces cinnamonensis. J Bacteriol 175:3511–3519 88. Vrilbloed JW, Zerbe-Burkhardt K, Ratnatilleke A, Grubelnik-Leiser A, Robinson JA (1999) Insertional inactivation of methylmalonyl coenzyme A (CoA) mutase and isobutyrylCoA mutase genes in Streptomyces cinnamonensis: influence on polyketide antibiotic biosynthesis. J Bacteriol 181:5600–5605 89.

One drawback often encountered with industrial production strains is a high barrier to genetic manipulation, which is not understood. Finally, genomics tools can also be used to obtain a comprehensive readout on cellular states in fermentation conditions. This allows the process engineer to correlate good and bad effects on production levels to molecular pathways in the cell, providing a more rational and direct approach to optimizing fermentation parameters. Some detailed examples of how genomic technologies can be used for process and strain improvement have been described [95].

Stanbury PF, Whitaker A (1986) Principles of fermentation technology. Pergamon Press, Oxford, UK 5. Steuer A, Ostrove JM, McEntire J (1996) Establishing cell banks under current good manufacturing practices. Biopharm 9:40–44 6. Brown MRW, Gilbert P (1995) Microbiological quality assurance screening and bioassay: a guide towards relevance and reproducibility of inocula. CRC Press, Boca Raton, FL 7. Demain AL, Davies JE (1999) Manual of industrial microbiology and biotechnology. American Society for Microbiology, Washington, DC 8.

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Advances in Biochemical Engineering Biotechnology Metabolic Engineering by Georg Gübitz, Alexander Bauer, Guenther Bochmann, Andreas Gronauer, Stefan Weiss

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